Effects of the amphiphilic peptides on membrane permeability of Acinetobacter baumannii
10.3969/j.issn.1671-8348.2015.09.003
- VernacularTitle:两性多肽结构影响鲍曼不动杆菌外膜通透性的实验研究*两性多肽结构影响鲍曼不动杆菌外膜通透性的实验研究
- Author:
Jie ZHANG
;
Yong CHEN
;
Ying ZENG
;
Xiujuan LI
- Publication Type:Journal Article
- Keywords:
bacteriophage;
endolysin;
amphiphilic peptides;
Acinetobacter baumannii;
membrane permeability
- From:
Chongqing Medicine
2015;(9):1162-1164,1167
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate effects of the amphiphilic peptides on membrane permeability of acinetobacter bauman-nii .Methods The LysAB3 and LysAB3-D (lack of amphiphilic peptides structure gene) was synthesized and inserted into the vec-tor pET28a(+ ) to construct the recombinant expression plasmid (pET28a-LysAB3 ,pET28a-LysAB3-D) .After expression in E . coli BL21(DE3) and purification with Ni2+-NTA Sepharose .Acinetobacter baumannii was observed by scanning electron microsco-py and fluorescence microscope ,pretreated with LysAB3 and LysAB3-D respectively .Results Under scanning electron microscopy , LysAB3-treated acinetobacter baumannii exhibited not only significant abnormalities ,including deep roughening of the cell surface , but also FITC readily accumulated in bacteria .It was different from LysAB3-D-treated .Conclusion These results indicate that the amphiphilic peptides structure increase membrane permeability of acinetobacter baumannii ,which helps LysAB3 degrade bacteria .
