Expression and function of miRNA-16 in peripheral blood monouclear cells of systemic lupus erythematosus patients

Zhihua Yin; Fenlian Guo; Huiqiong Zeng; Zhijun Cao; Xiuxia Luo; Zhizhong YE; Hualin Sun

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Expression and function of miRNA-16 in peripheral blood monouclear cells of systemic lupus erythematosus patients

VernacularTitle:miRNA-16在系统性红斑狼疮患者外周血中的表达及作用
Author: Zhihua YIN ; Fenlian GUO ; Huiqiong ZENG ; Zhijun CAO ; Xiuxia LUO ; Zhizhong YE ; Hualin SUN
Publication Type:Journal Article
Keywords: Systemic lupus erythematosus; miRNA-16; Peripheral blood mononuclear cells
From: Clinical Medicine of China 2015;(2):97-99,100
CountryChina
Language:Chinese
Abstract: Objective To investigate the expression of miRNA-16 in peripheral blood monouclear cells (PBMC)from systemic lupus erythematosus( SLE)patients. Methods Sixteen SLE patients who meet the diagnostic criteria of SLE revised in 1997 American rheumatology and 12 healthy individuals were selected as our subjects. Their peripheral blood were sampled. Total RNAs were extracted and purified. The level of miRNA-16 was determined by quantitative reverse transcription PCR( qRT-PCR). U6 was used as housekeeping control. The amount of target miRNA was normalized relative to the amount of U6(ΔCt =ΔCt miRNA-ΔCtU6 ). Relative expression levels were expressed as 2-ΔCt . Results The expression level of miRNA-16 in the SLE patients was 919. 87 ± 715. 45,significantly higher than that in the healthy control group(413. 6 3 ± 330. 69;t= -2. 497,P﹤0. 05). And miRNA-16 expression in SLE active group was 1 298. 79 ± 803. 79,significantly higher than that in SLE stable group(540. 95 ± 350. 15;t= -2. 445,P﹤0. 05). The level of miRNA-16 was related with AnuA (r=0. 669,P=0. 005),ESR(r=0. 608,P=0. 012)and SLEDAI(r=0. 530,P=0. 035). Conclusion The expression of miRNA-16 is high in SLE patients and it is related with SLE activity.