Prediction of the secondary structure and B-cell epitopes of Mycoplasma suis ORF5 protein
10.3969/cjz.j.issn.1002-2694.2015.03.014
- VernacularTitle:猪附红细胞体 ORF5蛋白的空间结构与B 细胞抗原表位预测
- Author:
Qing LYU
;
Si SHAN
;
Lijun WANG
;
Caifeng BA
- Publication Type:Journal Article
- Keywords:
Mycoplasma suis;
ORF5;
Secondary structure;
B-cell epitopes
- From:
Chinese Journal of Zoonoses
2015;(3):251-254,259
- CountryChina
- Language:Chinese
-
Abstract:
The objective of the study is to predict the spatial structure and B‐cell epitopes of Mycoplasma suis ORF5 pro‐tein .The secondary structure ,hydrophilicity ,flexible region ,antigenic index and surface probability were analyzed and predic‐ted by the Protean module in DNAStar software and B Cell Epitope Prediction Tools of IDEB ,then B‐cell epitopes were predic‐ted by aggregate analysis .Results showed that the secondary structure of Mycoplasma suis ORF5 protein was relatively regu‐lar ,in which the potential B cell antigenic epitopes were located at GGVDGGRD ,GMRLPEDSR ,and EGHPDLESAR .The methods of prediction of the secondary structure and B‐cell epitopes of Mycoplasma suis ORF5 protein may provide a new method for the study of M .suis immunogenicity ,and provides a new idea for the study on immunogenicity of pathogenic micro‐organisms .
