Processing Technology Optimization of Prepared Morinda Officinalis Based on Nystose Content
- VernacularTitle:基于耐斯糖含量优选制巴戟天炮制工艺
- Author:
Zhou CAN
;
Xiong HUI
;
Liao SHA
;
Fan LIN
- Publication Type:Journal Article
- Keywords:
Nystose;
HPLE-ELSD;
Prepared Morinda officinalis;
Processing technology
- From:
China Pharmacist
2015;(3):371-372,373
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To optimize the processing technology of prepared Morinda officinalis based on nystose content. Methods:Orthogonal experiments and the nystose determination method in Chinese Pharmacopoeia (2010 edition) were used to optimize the pro-cessing technology. A Welch AQ-C18(250 mm ×4.6 mm,5 μm)chromatographic column was applied with methanol-water(3∶97)as the mobile phase, the column temperature was at 25℃, the flow rate was 0. 8 ml·min-1 , the injection volume was 10μl, and the drift tube temperature was at 40℃ with low temperature atomization. Results:The linearity of nystose was good within the range of 0. 214 8-0. 644 4 mg·ml-1 and the recovery was 99. 5%(RSD=3. 5%,n=6). Conclusion: On the basis of nystose content determination, the optimal processing technology of prepared Morinda officinalis is as follows:liquorice of 4%, stir fry time of 15min and the boiling pot temperature of 200℃.
