The expression changes of miR-214 in hypoxic induced pulmonary artery smooth muscle cells of rats
10.3969/j.issn.1671-8348.2015.19.003
- VernacularTitle:不同低氧时间诱导的肺动脉平滑肌细胞m iR-214表达的动态变化
- Author:
Jiao YANG
;
Xuwei WU
;
Liyan ZHANG
;
Yi XIAO
;
Yuxuan ZHANG
;
Yanli LI
;
Xiqian XING
- Publication Type:Journal Article
- Keywords:
MiRNA-214;
anoxia;
pulmonary artery smooth muscle cells
- From:
Chongqing Medicine
2015;(19):2600-2601,2605
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the changes of microRNA‐214 (miR‐214) expression in rat pulmonary artery smooth mus‐cle cells (PASMCs) induced by different hypoxia time ,and lay the foundation to explore the effect and mechanism of regulation of miR‐214 on PASMCs proliferation .Methods The primary cultured PASMCs were cultured under hypoxic 0 h ,6 h ,12 h ,24 h ,48 h ,respectively .The real time quantitative PCR was used to detect miR‐214 expression in each group PASMCs .Results The ex‐pression of miR‐214 in hypoxia group PASMCs was sustained as time increased ,apart from hypoxic hypoxia 6h group and 0h group ,the expression of miR‐214 was no significant difference (P>0 .05);the expression of miR‐214 among other groups PASMCs was significantly different (P<0 .05) .Conclusion The expression of miR‐214 in PASMCs increased after induction of hypoxia .We speculated that miR‐214 may be involved in the regulation of hypoxia induced PASMCs proliferation .
