miR-100 expression in hepatocellular carcinoma tissues with fluorescent in situ hybridization
10.13315/j.cnki.cjcep.2015.02.017
- VernacularTitle:荧光原位杂交法检测肝细胞癌组织中miR-100的表达
- Author:
Hui ZHANG
;
Yangkun WANG
;
Wuling ZHU
- Publication Type:Journal Article
- Keywords:
hepatocellular neoplasms;
miR-100;
differentiation;
fluorescence in situ hybridization
- From:
Chinese Journal of Clinical and Experimental Pathology
2015;(2):184-185,186
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the expression and clinical significance of miR-100 in hepatocellular carcinoma ( HCC) . Methods LNA-modified miR-100 fluorescent probe was used to detect the formalin-fixed and paraffin-embedded tissues of 29 cases of HCC, while 10 cases of normal human liver tissues were used as the control group. Results Analysis of fluorescence in situ hybridization ( FISH) showed that, in all normal tissues miR-100 expression were positive, fluorescence signal located in the cytoplasm, while only 11 cases of HCC were positive, and there was a significant difference between both positive rates (100% vs 37. 9%, P<0. 01). In addition, with the degree of differentiation of HCC decreased, the expression of miR-100 showed a decreasing trend, positive rate of the well differentiation group and moderate differentiation group was significantly higher than the poor differentiation group ( P<0. 05 ) . Conclusion Detection of miR-100 expression in HCC tissue might be helpful to evaluate the pathologic grade of the tumors.