Expression levels and effects of thioredoxin reductase-1 on non-small cell lung cancer
10.13315/j.cnki.cjcep.2015.02.005
- VernacularTitle:非小细胞肺癌细胞中TrxR1的表达及其效应
- Author:
Fenghou GAO
- Publication Type:Journal Article
- Keywords:
lung neoplasms;
non-small cell lung cancer;
thioredoxin reductase-1;
target molecules;
cell proliferation
- From:
Chinese Journal of Clinical and Experimental Pathology
2015;(2):136-140,144
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To explore the expression of thioredoxin reductase-1 ( TrxR1 ) in non-small cell lung cancer and to observe the effects of TrxR1 on non-small cell lung cancer ( NSCLC) . Methods Immunohistochemistry was used to determine the expression of TrxR1 in 118 NSCLC tissues and 27 normal lung tissues, and to analyze its relationships with clinical pathological features. TrxR1 mR-NA levels were measured by quantitative RT-PCR in 13 cases of NSCLC and 4 cases of normal lung tissue. TrxR1 protein and its mR-NA levels were assessed by Western blotting and quantitative RT-PCR in normal lung epithelial cells and different lung cancer cell lines. After the silence TrxR1 expression in A549 cells, the changes of proliferation were compared. Results The data of immunohis-tochemistry and quantitative RT-PCR showed the overexpression of TrxR1 in NSCLC ( P <0. 05 ) . After using shRNA silencing of TrxR1 in lung cancer cells, cell proliferation changed significantly. Conclusion There are a high mRNA and protein levels of TrxR1 in NSCLC. TrxR1 may be a critical therapeutic target for the treatment in NSCLC.
