Optimization of the urine trace albumin and glycosylated hemoglobin measurements by response surface methodology
10.3969/j.issn.1673-4130.2014.21.029
- VernacularTitle:响应面分析法优化微量清蛋白及糖化血红蛋白检验条件
- Author:
Jianhong XU
- Publication Type:Journal Article
- Keywords:
response surface methodology;
trace albumin;
glycosylated hemoglobin
- From:
International Journal of Laboratory Medicine
2014;(21):2929-2930,2933
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the measurements of glycosylated hemoglobin and urine trace albumin based on the response surface methodology .Methods 60 cases of patients with diabetes were enrolled .All the patients were collected 3 mL fasting blood and 6 mL urine in the morning .The simple factors experiment was taken in different maintain temperatures and times .Based on the Box-Behnken design principles ,the temperatures and times were as the factors influencing .The response surface methodology was proceeding centered on the condition that the maintain temperature was 4 ℃ and the maintain time was 3 d .Results The best tem-perature for save urine and blood was 4 ℃ and the best time was 1 d .In this condition ,the measured value of glycosylated hemoglo-bin and urine trace albumin were mostly closed to the ideal value .Conclusion Using the response surface methodology ,the measure condition of glycosylated hemoglobin and urine trace albumin can be optimized .
