Correlation study between prokaryotic ubiquitin-like protein (Pup)-proteasome and drug resistance of clinical isolates of Mycobacterium tuberculosis
10.3969/j.issn.1000-484X.2014.11.001
- VernacularTitle:结核杆菌Pup-蛋白酶体系统与耐药结核杆菌耐药性的相关性研究
- Author:
Li HE
;
Ying LEI
;
Fang WU
;
Le ZHANG
;
Jiangdong WU
;
Xudong CAO
;
Bin ZHU
;
Bo WU
;
Ruishan LI
;
Yuqing ZHANG
;
Zhao WANG
;
Rui ZHUANG
;
Wenjuan LI
;
Chen LIANG
;
Chao FAN
;
Wanjiang ZHANG
- Publication Type:Journal Article
- Keywords:
Mycobacterium tuberculosis;
Drug-resistantance;
Prokaryotic ubiquitin-like protein ( Pup)-proteasome
- From:
Chinese Journal of Immunology
2014;(11):1441-1447,1451
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the correlation different drug-resistant mycobacterium tuberculosis clinical isolates of prokaryotic ubiquitin-like protein ( Pup )-proteasome of Pup, Dop, PafA, Mpa gene expression level and mycobacterium tuberculosis Pup-proteasome system with Xinjiang region widely popular drug-resistant mycobacterium tuberculosis in clinical isolates resistance.Methods:Total RNA of Mtb was extracted from cultured Mtb during the logarithmic phase in drug-susceptible strains in Xinjiang region,the clinical strains drug sensitive to INH,RFP,SM and EB respectively,and multidrug-resistant(MDR) strains.And then the purity of total RNA was identified.The expressing of Pup-proteasome relevant gene( Pup,Dop,Mpa,PafA) were quantified using SYBR GreenⅠqRT-PCR which aimed at finding the correlation between Mtb Pup-proteasome system and drug resistance of Mtb clinical isolates widespread in Xingjiang region by analyzing the expression of Pup, Dop, PafA, Mpa gene among different isolates.Results:Compared with the drug-senstive clinical isolates, mRNA expression level of Pup, Mpa gene was down-regulated in resistant M.tuberculosis clinical isolates INH ( INH-MTB) ,RFP ( RFP-MTB) ,SM ( SM-MTB) and EB ( EB-MTB) ,mRNA expression levels of genes in Dop and PafA was higher in resistant M.tuberculosis clinical isolates,the difference was statistically significant(P<0.05).Compared with MDR strain, the expression of Pup, Dop, Mpa gene were up-regulated different in the resistant M.tuberculosis clinical isolates isolates ,the expression of PafA gene was down-regulated different,the difference was statistically significant( P<0.05).Conclusion:The differentially expressed gene of Pup、Dop、PafA、Mpa gene in sensitive strains,INH-MTB,RFP-MTB,SM-MTB,EB-MTB and MDR strains.The Mycobacterium tuberculosis Pup-proteasome system.Therefore,the Pup-proteasome system have association with the drug resistance of Mtb strains widespread in Xinjiang region.
