TypeⅠ IFN-mediated enhancement of anti-osteosarcoma cytotoxicity of human gammadelta T cells

VernacularTitle:Ⅰ型IFN体外增强人γδT 细胞对骨肉瘤的杀伤作用
Author: Zhaoxu LI ; Ruiying WANG ; Jicun TANG
Publication Type:Journal Article
Keywords: Osteosarcoma; γδT cell; IFN; Cytotoxicity activity; Immunotherapy
From: Chinese Journal of Immunology 2014;(11):1533-1535,1542
CountryChina
Language:Chinese
Abstract: Objective:To investigate the effect of typeⅠIFN on the cytotoxic activity ofγδT cells from peripheral blood mono-nuclear cells ( PBMCs) of healthy donors and osteosarcoma patients stimulated by zoledronate ( Zol) and IL-2 against OS.Methods:PBMCs from healthy donors and osteosarcoma patients were stimulated with Zol+IL-2 or Zol+IL-2+typeⅠIFN,respectively.After 14 days of culture,ex vivo expandedγδT cells were assessed by flow cytometry.γδT cell cytotoxicity against target cells was analyzed using a standard lactate dehydrogenase release assay.IFN-γsecreted fromγδT cells was determined by ELISA kits.Results:γδT cells from PBMCs of healthy donors and patients with OS were selectively expanded by Zol+IL-2 or Zol+IL-2+typeⅠIFN in vitro,respectively, and showed cytotoxicity against OS.In addition,γδT cells pre-treated by TypeⅠIFN showed significantly higher cytotoxicity against OS cells.Conclusion:Type I IFN can enhance humanγδT cells’ cytotoxic activity against OS.