Effects of α-enolase silencing on drug resistance in drug resistant cell line K562/A02
10.3969/j.issn.1001-1978.2014.11.008
- VernacularTitle:干扰α烯醇化酶对耐药细胞株K562/A02耐药性的影响
- Author:
Xue GAO
;
Zhou YE
;
Kexiong WU
;
Dongmei FAN
;
Ming YANG
;
Yanjun ZHANG
;
Yizhi ZHANG
- Publication Type:Journal Article
- Keywords:
human chronic myeloid leukemia;
drug resistance;
K562/A02;
cell growth;
eno1;
MDR1
- From:
Chinese Pharmacological Bulletin
2014;(11):1521-1525,1526
- CountryChina
- Language:Chinese
-
Abstract:
Aim Drug resistance is one of the major hinders on cancer treatments. α-enolase ( eno1 ) was closely related to the generation and development of drug resistance. This article aims to study the effect of eno1 on cell growth and drug resistance in human chro-nic myeloid leukemia cell line K562/A02 . Methods We screened three eno1 stable silencing cells K562/A02-sheno1 and its control cells K562/A02-shcon. Cell count assay was performed to test cell growth, MTT assay was used to test cell proliferation, flow cytometry was used to test the intra-cellular Rho123 content, the expression of genes were tested by real-time PCR assay and western blot assay on mRNA level and protein level, respectively. Results eno1 was o-ver-expressed in K562/A02 cells and its expression was increased by ( 2. 85 ± 0. 56 ) times and ( 1. 43 ± 0. 05 ) times on mRNA level and protein level com-pared to K562 cells. However, there was no difference in cell growth rate between K562/A02 cells and K562 cells. K562/A02-sheno1 cells showed lower cell growth rate and higher drug sensitivity to anti-cancer drugs taxol and doxorubicin. Moreover the Rho123 content was increased in K562/A02-sheno1 cells. The expression of MDR1 decreased in both mRNA level and protein level in K562/A02-sheno1 cells. Conclusion eno1 silencing could suppress cell growth, reverse drug resistance and increase its drug sensitivity in K562/A02 cells, and the mechanism was associated with the MDR1 gene.
