Progesterone protects neurons against impairment induced by Aβactivated astrocytes
10.3969/j.issn.1001-1978.2014.11.012
- VernacularTitle:孕酮抑制Aβ诱导星形胶质细胞活化所致神经元损伤
- Author:
Jie WU
;
Honghai WU
;
Yang YU
;
Yabin QIN
;
Xiaolei HAN
;
Yanning HOU
- Publication Type:Journal Article
- Keywords:
Alzheimer ’ s disease;
β-amyloid;
astro-cytes;
neurons;
progesterone;
TNF-α;
IL-1β;
NF-κB
- From:
Chinese Pharmacological Bulletin
2014;(11):1539-1542,1543
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of progester-one ( PROG) in protecting the neurons against impair-ment induced by the Aβ1-42 activated astrocytes, and the underlying molecular mechanism. Methods The astrocytes were divided into 5 groups: control, Aβ, and Aβplus PROG groups treated with 3 different con-centrations of progesterone for 24h. Then, Aβand pro-gesterone were removed, and neurons were co-cultured with the treated astrocytes. MTT assay was used to e-valuate the viability of cultured neurons; ELISA was used to detect the levels of IL-1βand TNF-αin culture media of astrocytes; immunofluorescence and Western blot were performed to detect the activation of NF-κB in astrocytes. Results PROG dose dependently pro-tected against Aβ1-42 activated astrocytes induced via-bility decrease in co-cultured neurons. Aβ induced release of IL-1β and TNF-α from astrocytes, and in-crease of NF-κB activity was abolished by progesterone treatment. Conclusion PROG protects the neurons through inhibiting the reactivity of astrocytes, and the underlying mechanism involves the NF-κB signal trans-duction.
