Correlation between fatty acid receptor GPR120 and glucose transporter 4
10.3969/j.issn.1000-484X.2014.11.008
- VernacularTitle:脂肪酸受体GPR120与葡萄糖转运蛋白4的关系
- Author:
Dandan LIU
;
Qiuxia YU
;
Huijuan ZHANG
;
Xiaoyan JIANG
- Publication Type:Journal Article
- Keywords:
Free fatty acids;
Glucose transporter 4;
G-protein coupled receptor 120;
Insulin resistance
- From:
Chinese Journal of Immunology
2014;(11):1477-1479,1484
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the correlation between G protein-coupled receptor 120 ( GPR120 ) and glucose transporter 4 (GLUT4) in 3T3-L1 cells.Methods:3T3-l1 cells were induced for differentiation,GRP120 mRNA was detected by RT-PCR and Oil red O was used to determine fat expression.GPR120 expression was knocked down by a specific siRNA in 3T3-L1cells.3T3-L1 cell that were tranfected with GPR120 siRNA were incubated with palmitic acid for 24 hours.Then,real-time PCR and Western blot were used to detect the expression of GLUT4 mRNA and protein.Results: Induced differentiation led to GPR120 overexpression in 3T3-L1 cells ( P<0.05 ).GPR120 knockdown resulted in reduction of both lipid volume and number in 3T3-L1 cells.Furthermore,GPR120 knockdown decreased GLUT4 mRNA and protein levels in 3T3-L1 cells (P<0.05,respectively).Conclusion:GPR120 affects the expression level of GLUT4 in insulin signaling pathway,suggesting its involvement in the initiation of insulin resist-ance.
