Immune effect of Aeromonas hydrophila inactivated vaccine on carassius gibelio

VernacularTitle:嗜水气单胞菌灭活疫苗对银鲫的免疫效果研究
Author: Huili MAO ; Jianyi GUAN ; Wenxu HE ; Jing KANG
Publication Type:Journal Article
Keywords: Aeromonas hydrophila; Tissue section; Inactivated vaccine; Immunological indicators; Protection rate
From: Chinese Journal of Immunology 2014;(11):1499-1503,1507
CountryChina
Language:Chinese
Abstract: Objective:In order to find a safe and healthy way to control fish diseases caused by Aeromonas hydrophila,the immune effect of Aeromonas hydrophila inactivated vaccine on Carassius gibelio was studied.Methods: Three groups were randomly made by each of 10 fishes.Injection groups was made intraperitoneally ( i.p.) with 200 μl, and soaked group with 3 L volumes of inactivated vaccine suspensions respectively while the control group with same volumes of saline.Tissues and blood samples of carassius gibelio were collected after the second vaccination.Then the histopathological, immunological indicators and survival percentage were detected.Results:The relative survival percentage of two groups was 90%and 60%respectively.The serum enzymes of injection and soaked group showed significant differences contrasted with the control group.Histopathological studies revealed that the important tissues have been damaged obviously after the bacterium challenge, while the experiment groups were no damage.Conclusion:Therefore the inactivated vaccine could prevent carassius gibelio diseases that caused by A.hydrophila.