Comparison of BRAF mutation detection in patients with papillary thyroid microcarcinoma by ARMS and direct sequencing

VernacularTitle:直接测序法与ARMS法检测甲状腺乳头状微小癌组织中BRAF基因突变的比较
Author: Xiumei DUAN ; Yongliang TENG ; Lingling TONG ; Zhuang TIAN ; Mo SUN ; Haiying WANG ; Meishan JIN
Publication Type:Journal Article
Keywords: Papillary thyroid microcarcinoma; BRAF gene mutation; DNA sequencing; Scorpions amplification refractory mutation system
From: Chinese Journal of Immunology 2014;(11):1514-1516,1522
CountryChina
Language:Chinese
Abstract: Objective:To investigate the sensitivity and the specificity of scorpions amplification refractory mutation system ( ARMS) in comparing with that of direct DNA sequencing in the detection of BRAF gene mutations in patients with papillary thyroid microcarcinoma.Methods:Direct sequencing and ARMS were used simultaneously to detect BRAF mutation status in 56 patients with PTMC.Results:BRAF mutations were identified in 46 cases with a mutation rate of 82.9%by ARMS,while in 18 cases with a mutation rate of 32.1%by direct sequencing.Besides,the sensitivity of ARMS was 100%and that of direct sequencing was 39.1%.There were significant differences of both mutation rate and sensitivity between two methods ( P<0.01 ).Conclusion: Compared to direct sequencing,ARMS gains a higher sensitivity in the detection of BRAF mutations in samples with tiny lesions.