Hydrogen sulfide inhibits cardiomyocyte apoptosis induced by acute myocardial ischemia in isolated perfused rat heart
10.3969/j.issn.1001-1978.2014.11.013
- VernacularTitle:硫化氢抑制离体灌流大鼠急性心肌缺血所致心肌细胞凋亡研究
- Author:
Yinghua XIE
;
Yanshan MA
;
Nan ZHANG
;
Jianxin ZHANG
- Publication Type:Journal Article
- Keywords:
hydrogen sulfide;
acute myocardial ische-mia;
Langendorff;
rat;
cardiomyocyte apoptosis;
cardio-protection
- From:
Chinese Pharmacological Bulletin
2014;(11):1543-1546,1547
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate whether hydrogen sul-fide ( H2 S ) inhibits cardiomyocyte apoptosis induced by acute myocardial ischemia in isolated perfused rat heart. Methods The myocardial ischemia injury model was replicated with Langendorff isolated perfused rat heart, and the left anterior descending coronary ar-tery was ligated for 4 h. 40 male SD rats were divided into five groups randomly: sham group, ischemia group, and NaHS groups (5,10,20μmol·L-1). The segmental heart samples were used for HE staining. Cardiomyocyte apoptosis was detected with TUNEL as-say. The expressions of caspase-3 and Cyt-C in hearts were determined with Western blot analysis. Results Myocardial cells were found to show serious disorder and coagulated zonal necrosis under light microscope, the apoptotic rate of cardiomyocytes and the expression of caspase-3 and Cyt-C were significantly increased af-ter ischemia for 4h. Perfusion of NaHS resulted in more clear cell morphology and milder pathologic chan-ges of myocardiocytes according to the HE staining a-nalysis, and the significant decrease of expression of Cyt-C. After perfusion of 10,20 μmol·L-1 NaHS,the apoptotic rate of cardiomyocytes and the expression of caspase-3 were significantly decreased. Conclusion H2 S has certain protective effects on acute myocardial ischemic injury in isolated perfused rat heart via inhibi-ting cardiomyocyte apoptosis.
