Significance of dynamic detection of free DNA in hypertensive cerebral hemorrhage

VernacularTitle:高血压脑出血患者脑脊液中游离 DNA 动态检测的意义
Author: Jiafeng NIU ; Lei JIA ; Xue LI ; Zongxiang LI ; Qing LI ; Debao ZHOU
Publication Type:Journal Article
Keywords: cell free DNA; intracerebral hemorrhage; brain edema; intracranial infection
From: International Journal of Laboratory Medicine 2014;(20):2779-2780,2783
CountryChina
Language:Chinese
Abstract: Objective To study the relationship between the dynamic change of free DNA in cerebrospinal fluid (CSF)and the tissue injury in the patients with hypertensive intracerebral hemorrhage.Methods 54 cases of hypertensive intracerebral hemor-rhage were divided into 3 groups according to the glasgow coma scale(including 17 mild cases,21 moderate cases and 16 severe ca-ses).2 mL of CSF was collected for extracting free DNA on 1,3,7,15 d after stroke onset.The free DNA level was measured by the fluorescent real-time PCR.Results The free DNA level in the severe group was significantly higher than that in the mild and mod-erate groups.The free DNA level in the abnormal intracranial pressure group was higher than that in the normal intracranial pres-sure group;the intracranial infection group was higher than the non-infection group.Conclusion The free DNA level has certain value for diagnosing the craniocerebral injury in hypertensive intracerebral hemorrhage and is conducive to monitor the occurrence of postoperative complications.