Comparison of hepatitis C virus RNA and antibody detection method in population screening and its application
10.3969/j.issn.1673-4130.2014.20.039
- VernacularTitle:丙型肝炎病毒核酸和抗体检测方法在人群筛查中的比较及应用
- Author:
Hongyan ZHU
;
Sheng BI
;
Xi YANG
;
Zheng LI
;
Yunmin XU
- Publication Type:Journal Article
- Keywords:
hepatitis C virus antibodies;
hepatitis C virus RNA;
enzyme-linked immunosorbent assay;
real-time PCR
- From:
International Journal of Laboratory Medicine
2014;(20):2811-2812,2815
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the application of hepatitis C virus RNA and antibody detection method in population screening.Methods The colloidal gold rapid test method and the enzyme-linked immunosorbent assay (ELISA)were adopted to detect hepatitis C virus (HCV)antibodies,and the real-time quantitative PCR (RT-PCR)was adopted to detect HCV-RNA viral load.Results (1)Among 539 samples,266 cases were antibody negative and 263 cases were antibody positive.(2)Among 67 cases in the HCV-RNA viral load <103 IU/mL group,60 cases were HCV antibody positive by ELISA and 30 cases were HCV antibody positive by colloidal gold rapid test.Among 208 cases in the HCV-RNA viral load ≥ 103 IU/mL,199 cases were antibody positive by ELISA,but only 181cases were antibody positive by the colloidal gold rapid method.Other 6 cases of were 2 kinds of antibody negative had the HCV-RNA viral load ≥ 103 IU/mL.(3)208 cases of HCV-RNA viral load ≥ 103 IU/mL sample were divided in-to four groups.GGT,ALT and AST were statistically significantly different P <0.05),while ALB and S/CO values hadno statisti-cal difference (P >0.05).Conclusion In order to reduce the missed diagnosis rate and diagnose hepatitis C as early as possible,the above laboratory detection methods should be jointly applied and the comprehensive analysis should be conducted in population screening.
