Calcification-resistant Effect of Surface-modified Biologic Arteries by Sulfonated Polyethyleneoxide.
- Author:
Hyoung Mook KIM
1
;
Man Jong BAEK
;
Kyung SUN
;
Sung Yeoll LEE
;
Song Ahm LEE
;
Kwang Taik KIM
;
In Sung LEE
;
Won Gyu LEE
;
Gee Dong PARK
;
Young Ha KIM
Author Information
1. Department of Thoracic and Cardiovascular Surgery, College of Medicine, Korea University. Seoul, Korea.
- Publication Type:Original Article
- Keywords:
After implantation of 3 weeks;
PEO-SO3 group showed significantly less depositions
- MeSH:
Adult;
Animals;
Arteries*;
Calcium;
Carotid Arteries;
Dogs;
Glutaral;
Humans;
Polyethylene Glycols*;
Propane;
Reoperation
- From:The Korean Journal of Thoracic and Cardiovascular Surgery
1999;32(11):989-997
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Calcific degeneration is the major cause of clinical failure of glutaraldehyde (GA) crosslinked bioprosthetic tissues implanted in the body and necessitates the reoperation or causes death. Surface modification of biologic tissues using sulfonated polyethyleneoixde (PEO-SO3) has been suggested to significantly enhance blood compatibility, biostability and calcification-resistance by means of the synergistic effect of highly mobile and hydrophilic PEO chains and electrical repulsion of negatively charged sulfonate groups. This study was designed to evaluate the anticalcification effect of surface-modification of biologic arteries by direct coupling of PEO-SO3 after GA fixation and changes of calcification according to the implantation period through the quantitative investigation of the deposited calcium and phosphorous contents of the biologic arterial tissues in the canine circulatory implantation model. MATERIAL AND METHOD: Total of 16 fresh canine carotid arteries were harvested from eight adult dogs and divided in to GA group(n =8) and PEO-SO3 group(n=8). Sulfonation of diamino-terminated PEO was performed using propane sultone. Canine carotid arteries were only crosslinked with 0.65% GA solution in GA group and modified by direct coupling 5% PEO-SO3 solution after GA crosslinkage for 2 days and stabilized by NaBH4 solution for 16 hours in PEO-SO3 group. In both groups the resected segment of bilateral carotid arteries were reconstructed. Reconstructed segments of the two groups were analysed the quantities of calcium and phosphorous contents after 3(n=4) and 6(n=4) weeks in vivo. RESULT: After implantation of 3 seeks, PEO-SO3 group showed significantly less depositions.
