Associations of -2548A/G polymorphism in leptin gene and Gln223Arg polymorphism in leptin receptor gene with asthma and metabolic syndrome
10.3969/j.issn.1000-484X.2014.11.006
- VernacularTitle:瘦素基因-2548 A／G和瘦素受体基因Gln223 Arg位点多态性与哮喘和代谢综合征的关系
- Author:
Yunxiao LI
;
Xia JI
;
Junjie GAO
- Publication Type:Journal Article
- Keywords:
Leptin;
Leptin receptor;
Gene polymorphism;
Asthma;
Metabolism syndrome
- From:
Chinese Journal of Immunology
2014;(11):1467-1471,1476
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the relationship between polymorphism of leptin gene -2548A/G and leptin receptor gene ( LEPR) Gln223Arg and asthma and metabolic syndrome.Methods: 82 asthma-combined metabolic syndrome patients,114 asthma patients,100 metabolic syndrome patients and 96 normal controls were conducted.The polymerase chain reaction and restriction fragment length polymorphism ( PCR-RFLP) analysis were performed to investigate the polymorphism of leptin gene -2548A/G and LEPR gene Gln223Arg site.In addition, asthma was graded into mild AS and mod-severe AS according to lung function.Then the associations between the polymorphism of leptin gene -2548A/G and LEPR gene Gln223Arg and different grades of asthma were performed.Results:①The biochemical indicators were different compared between each group.②The genotype and allele frequencies in leptin gene polymorphism -2548A/G were significantly difference between metabolic syndrome patients(P=0.047 and 0.046), asthma-combined metabolic syndrome patients( P=0.038 and 0.044) ,mod-severe asthma patients( P=0.019 and 0.028) and control group.③There was a significant difference of genotype and allele frequencies in LEPR gene Gln223Arg between metabolic syndrome patients and controls(P=0.037 and 0.023);between metabolic syndrome patients and asthma patients(P=0.000 and 0.000).There was a significant difference of allele frequencies in LEPR gene Gln223Arg between asthma-combined metabolic syndrome patients and asthma(P=0.032) .Conclusion: Polymorphisms of the leptin gene -2548A/G site may be associated with metabolic syndrome and mod-severe asthma.Polymorphisms of the LEPR gene Gln223Arg site may be only associated with metabolic syndrome.The two genes would be the candidate genes in early prevention and control.
