Screening and identification of siRNA-mediated TNF-α expression in synovial cells of Human
10.3969/j.issn.1671-8348.2014.31.037
- VernacularTitle:靶向抑制滑膜细胞TNF-α表达的siRNA筛选及鉴定
- Author:
Xiaolong SHEN
;
Qi LIAO
;
Liang HAO
- Publication Type:Journal Article
- Keywords:
tumor necrosis factor-alpha;
RNA,small interfering;
synovial cells of human;
traumatic arthritis
- From:
Chongqing Medicine
2014;(31):4230-4231,4234
- CountryChina
- Language:Chinese
-
Abstract:
Objective To design and identify small interfering RNA (siRNA) targeting tumor necrosis factor‐α (TNF‐α) ex‐pression ,siRNAs were electroporated into synovial cells of Human to screen those which can effectively suppress TNF‐α expres‐sion .Methods Three TNF‐α specific double stranded siRNA were designed targeting different regions of TNF‐α mRNA(compared with negative control group) .RT‐PCR and Elisa were applied to detect TNF‐α ,mRNA expression and the secretion of TNF‐α in cell supernatant ,respectively .Results Two of the three customized TNF‐α siRNA could inhibit the expression of TNF‐α mRNA in synovial cells of Human (P< 0 .01) .At the same time ,the secretion of TNF‐α decreased in cell supernatant ,the difference was sig‐nificant statistically compared with the control group(P< 0 .01) .Conclusion TNF‐α siRNA can be successfully designed and syn‐thesized ,which can specifically and effectively suppress TNF‐α mRNA expression .
