Establishment and optimization of a method to extract miRNAs from plasma
10.7644/j.issn.1674-9960.2014.09.019
- VernacularTitle:血浆miRNA快速制备方法建立和优化
- Author:
Hanwei LI
;
Yiran ZHONG
;
Hanjiang FU
;
Yi TIE
;
Jie ZHU
;
Guiying LI
;
Xiaofei ZHENG
- Publication Type:Journal Article
- Keywords:
plasma;
miRNAs;
extract;
recombinant ribonuclease inhibitor
- From:
Military Medical Sciences
2014;(9):733-736,740
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop and optimize a new method to extract miRNAs from plasma.Methods miRNAs were extracted from plasma by mixing it with the extraction solution that contained surfactant and by heating .Then the ribonuclease inhibitor was added into the extraction to prevent RNAs from degradation .The expression level of each miRNA was detected by real-time quantitative PCR in oder to evaluate the feasibility of this method .Results A method which extracted miRNAs from plamsa in just one step was established .The specificity , reproducibility and stability of this method have been demonstrated by real-time quantitative PCR .Conclusion The one-step method is simple , inexpensive , and plasma-saving.It seems like a new method for clinical examination of miRNAs from plasma .
