Application evaluation of immunophenotyping in monoclonal gammaglobulinemia
10.3969/j.issn.1673-4130.2015.01.027
- VernacularTitle:免疫分型在单株丙球蛋白血症中的应用评估
- Author:
Ping WANG
;
Luping ZHANG
;
Lisong SHEN
- Publication Type:Journal Article
- Keywords:
monoclonal gammaglobulinemia;
Ig quantitation;
immunophenotyping;
immunofixation electrophoresis
- From:
International Journal of Laboratory Medicine
2015;(1):64-65,68
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the clinical value of immunophenotyping and quantitative analysis of serum immunoglobulin in the diagnosis and typing of monoclonal gammaglobulinemia.Methods 118 serum samples were collected from the patients with monoclonal gammaglobulinemia and performed the serum protein electrophoresis(SPE),serum immunofixation(IF)electrophoresis, immunoglobulins(Ig)quantitation and serum light chain κ,λdetection.The analysis was conducted aiming at the patients with im-munophenotyping positive and the Ig quantitation negative.Results 56 cases showed immunophenotyping positive and the immuno-globulin quantitation negative,among them,6 cases were free light chain type (κlight chain in 2 cases andλlight chain in 4 cases), 1 case was the non-secretion type;62 cases showed immunophenotyping positive and the Ig quantitation positive.Conclusion The IF technique has an important significance for the diagnosis and typing of monoclonal gammaglobulinemia,its diagnosis can be com-bined with the other laboratory tests of serum Ig and light chain quantitative detection.
