Establishment of mice Kawasaki disease model by Lactobacillus casei cell wall extract
10.3969/j.issn.1673-4130.2014.21.002
- VernacularTitle:干酪乳杆菌细胞壁成分构建川崎病动物模型
- Author:
Baidu ZHANG
;
Xiaoqiong GU
;
Jianqing XIA
;
Yong HUANG
;
Mingguang ZHAO
;
Lijuan BAO
- Publication Type:Journal Article
- Keywords:
Kawasaki disease;
Lactobacillus casei;
cell wall;
mice
- From:
International Journal of Laboratory Medicine
2014;(21):2867-2868,2871
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish mice models of Kawasaki disease by Lcatobacillus casei cell wall extract(LCWE) ,in order to provide experimental materials for follow-up study .Methods LCWE group was given LCWE 0 .1 mL per mouse(containing 200 mg LCWE) via abdominal intramuscular injection .PBS group was given 0 .1 mL PBS per mouse .Orbital blood samples were collected respectively at the 1st ,3rd and 28th day after administration .The heart ,lung ,liver ,kidney ,spleen and other organs tissue samples were collected for pathological section ,HE staining .The histopathological changes were investigated ,and the routine blood test was proceeded .Results The WBC of LCWE group showed a trend of rising at the 1st ,3rd and 28th day after administration .PLT and MPV of LCWE group increased at the 3rd day after administration ,which returned to normal levels at the 28th day after adminis-tration .The pathological section showed the blood vessel walls of heart tissue enlargement ,surrounded and infiltrated by inflamma-tory cells infiltration ,the atheromatous plaque in blood vessels occasionally .Conclusion The study established mice model of Ka-wasaki disease successfully .
