Effect of Polysaccharide from Panax japonicus on Hepatic Cell Injury
10.3969/j.issn.1005-5304.2014.11.018
- VernacularTitle:竹节参多糖对肝细胞损伤的影响
- Author:
Yue QIN
;
Changcheng ZHANG
;
Ting WANG
;
Ding YUAN
;
Chaoqi LIU
- Publication Type:Journal Article
- Keywords:
polysaccharide of Panax japonicus;
steatosis;
palmitic acid;
endoplasmic reticulum stress
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(11):59-61,62
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the protective effects of polysaccharide from Panax japonicus on free fatty acid in different parts of hepatic cell injury. Methods Polysaccharide of Panax japonicus was prepared through different concentrations of ethanol precipitation and was named as 30%polysaccharide component (pc), 60% pc and 90% pc. Palmitic acid (PA) was used to induce a cellular model of steatosis in HepG2 cells in order to screen the intervention viability of polysaccharide of Panax japonicus. MTT method was used to detect cell viability, and Oil Red O staining was used to demonstrate steatosis. Total RNA was extracted to detect the expression level of the relevant genes. Results MTT results showed that the 30% pc significantly increased cell viability compared with the model group;Oil Red O staining showed that the number of intracellular lipid droplets was significantly reduced in the 30% pc compared with the model group;RT-PCR results showed that expressions of the endoplasmic reticulum stress-related gene glucoese-regulated protein, CCAAT enhancer binding protein homologous protein and TNF-αwere significantly lower in the 30% pc compared with the model group, and there was no significant difference compared with normal control group. Conclusion The 30%ethanol precipitation fraction of polysaccharide from Panax japonicus significantly reduced PA-induced steatosis in HepG2 cells. Its mechanism was possibly realized through intervention in endoplasmic reticulum stress-related response.
