Cardamonin induces apoptosis and expression of apoptosis-related proteins in K562 cells
10.3969/j.issn.1671.7856.2014.010.002
- VernacularTitle:小豆蔻明诱导 K562细胞凋亡及其相关凋亡蛋白的表达
- Author:
Wo ZHAI
;
Yiping SHEN
;
Yanjing SHEN
;
Zhengqing LOU
;
Zhishan DING
- Publication Type:Journal Article
- Keywords:
Cardamonin;
K562 cells;
Apoptosis,PTEN,p-Akt,NF-κB,Bcl-2
- From:
Chinese Journal of Comparative Medicine
2014;(10):7-11,17
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the apoptotic effect of cardamonin on K562 cells and its relationship with the expressions of PTEN, p-Akt, NF-κB and Bcl-2.Methods K562 cells were treated with cardamonin for 48 h, and the following tests were performed:(1) The cell morphology was observed by light microscopy.(2)IC50 of the K562 cells was dtermined by MTT test.(3) The apoptosis rate was detected by flow cytometry.(4) The expressions of Bcl-2 and Bax mRNA were detected a by RT-PCR.(5) The expressions of PTEN, p-Akt, NF-κB and Bcl-2 proteins were detected by Western blot.Results Obvious apoptosis was observed in the K562 cells after treated with cardamonin for 48 h.MTT assay indicated that the proliferation of K562 cells was obviously inhibited in a dose-and time-dependent manner. Comparing with the blank group, the early apoptosis rate and expression of Bax mRNA were significantly increased.At the same time, the expression of Bcl-2 mRNA was significantly decreased.All of them presented a dose-dependent manner. The expression of PTEN obviously increased with the increasing dose of cardamonin and the expressions of p-Akt, NF-κB and Bcl-2 were decreased.Conclusions Cardamonin promotes the apoptosis in K562 cells in a dose-dependent manner by increasing the expression of PTEN and decreasing the expressions of p-Akt, NF-κB, and Bcl-2.
