Immune stimulation research of Portulaca oleracea L.polysaccharides on cervical tumor-bearing mice
10.3969/j.issn.1000-484X.2014.10.010
- VernacularTitle:马齿苋多糖对荷宫颈癌小鼠免疫刺激活性的研究
- Author:
Rui ZHAO
;
Xu GAO
;
Xingyue SHAO
- Publication Type:Journal Article
- Keywords:
Portulaca oleracea L;
Polysaccharide;
Tumor-bearing mice;
Immunity
- From:
Chinese Journal of Immunology
2014;(10):1344-1348,1368
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the immune stimulation effect of Portulaca oleracea L.polysaccharide ( POL-P3b) on cervical tumor-bearing mice.Methods:Using graft U14 cervical cancer mouse model ,and all the mice were randomly divided into tumor-bearing control group,cyclophosphamide (CTX) group and purslane polysaccharide low (L) (POL-P3b(L)) group and high dose of POL-P3b (POL-P3b(H)) group.After continuous treating 13 d,the thymus index and spleen index were determined; cellular immune function in mice was evaluated by ConA inducing mice spleen lymphocyte transformation test.The cytokines content of IL-2,IFN-γ,IL-10 and IL-4 secreted by spleen cells was detected using ELISA method.Indoleamine 2,3-2 oxidase ( IDO) expression in tumor tissue was detected by immunohistochemistry and Western blot method.Results:POL-P3b could obviously improve immune organ weight of tumor-bearing mice and enhance the cellular immunity function.POL-P3b could reduce the level of IL-4 and IL-10 secreted by spleen cells , and could promote the secretion of IL-2 and IFN-γ.POL-P3b also could inhibit the expression of IDO in tumor tissues ,and the effect of high dose group was more obvious.Conclusion:The antitumor mechanism of POL-P3b might be related to increase the body′s immune function and reduce the immune escape.
