The angiogenesis effect and mechanism of puerarin in human endothelial cells
10.3969/j.issn.1673-4130.2014.19.002
- VernacularTitle:葛根素对人脐静脉内皮细胞的促血管生成作用及机制的研究
- Author:
Jie ZHANG
;
Miaotian GONG
- Publication Type:Journal Article
- Keywords:
stroke;
puerarin;
phosphorylase nitric oxide synthase
- From:
International Journal of Laboratory Medicine
2014;(19):2579-2580,2583
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of puerarin on proliferation ,invasion ,migration and tube formation in human endo-thelial cells and its possible mechanism .Methods The effect of puerarin on cell proliferation was determined using methyl thiazolyl tetrazolium(MTT) assay .Invasion was evaluated with transwell chamber .Migration was performed by the wound healing method . Endothelial tube formation was performed by tube formation assay .The expressions of phosphorylase Akt(p-Akt) and phosphoryl-ase nitric oxide synthase(p-eNOS) protein were determined by western blot .Results Puerarin promote the proliferation ,invasion , migration and tube formation of human endothelial cells(P<0 .05) .The expression of p-Akt and p-eNOS were increased signifi-cantly(P<0 .05) .Conclusion Puerarin can promote the proliferation ,invasion ,migration and tube formation of human endothelial cells .Up regulation of p-AKT and p-eNOS protein may be one of its mechanisms .
