Study of Effect of CtBP2 Knockout through shRNA on Proliferation of Prostate Cancer Cells
10.3969/j.issn.0253-9896.2014.10.005
- VernacularTitle:shRNA沉默CtBP2基因对前列腺癌细胞的增殖作用
- Author:
Yan LIU
;
Yong XU
;
Chao GAO
;
Zhihong ZHANG
- Publication Type:Journal Article
- Keywords:
prostatic neoplasms;
cell line,tumor;
RNA interference;
cell proliferation;
CtBP
- From:
Tianjin Medical Journal
2014;(10):977-979,980
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of shRNA-CtBP2 on the growth of prostate cancer PC3 cells. Methods There were three experimental groups in this study,which include blank control group, empty plasmid transfected group and transfected shRNA group. CtBP2 mRNA sequence is targeted by 3 pairs of designed interfering shRNA to built shRNA-Ct-BP2 recombinant plasmid then it is transfected into PC3 cells. Reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot assays were used to detect the transcription and expression levels of CtBP2 mRNA and protein, respective-ly. PC3l proliferation was measured by MTT assay. Results Builting shRNA-CtBP2 recombinant plasmid and transfect-ing PC3 cells were successful. Transcription and expression levels of CtBP2 mRNA and protein were significantly decreased in shRNA-CtBP2 transfected PC3 cells. After CtBP2 silencing, cell proliferation was blocked in the shRNA-CtBP2 cells compared to that of blank control group (P<0.01). Conclusion shRNA-CtBP2 could significantly inhibit CtBP2 expres-sion, suppress the growth of PC3 cells, which suggests that CtBP2 may be a new target for PCa gene therapy.
