Expression profile of microRNAs under different lever of HER-2 in human breast cancer MCF-7 cells

VernacularTitle:人乳腺癌MCF-7细胞在不同HER-2水平miRNAs表达谱检测
Author: Chengchen XU ; Guilu ZHU ; Xian WANG ; Zhengsheng WU ; Qiang WU ; Rong QIN
Publication Type:Journal Article
Keywords: breast neoplasm; miRNAs; HER-2
From: Chinese Journal of Clinical and Experimental Pathology 2014;(9):967-970,974
CountryChina
Language:Chinese
Abstract: Purpose To screen out miRNA expression profile related to HER-2 via observing the differential expression of miRNA ex-pression of human breast cancer cells MCF-7 and MCF-7/HER-2. Methods After cultivating human breast cancer cells MCF-7 and MCF-7/HER-2, the expression profile of miRNAs was detected by miRNA microarrays. Results Compared to MCF-7 cells, two hundreds and seventeen HER-2 related miRNAs were identified from breast cancer MCF-7/HER-2 cells, including 123 up-regulated and 94 down-regulated miRNAs. Of significant differentialy expressed miRNAs, hsa-miR-141 and hsa-miR-1299’s target genes have multiple biological functions. Conclusion The differentially expressed miRNAs under different lever of HER-2 in human breast cancer MCF-7 cells were obtained, which may provide a basis for studying HER-2 functions and roles in breast cancer.