Determination of 23-Acetate Alisol B in Longdan Xiegan Honey Pills by HPLC
- VernacularTitle:HPLC法测定龙胆泻肝丸（大蜜丸）中23-乙酰泽泻醇B的含量
- Author:
Shun ZHAO
;
Hui SUN
;
Ye DING
;
Jinfeng ZHENG
;
Wenli LI
;
Li LIU
;
Ling LI
;
Yang XI
- Publication Type:Journal Article
- Keywords:
Longdan Xiegan honey pills;
23-Acetate alisol B;
HPLC;
Determination
- From:
China Pharmacist
2014;(12):2149-2150,2151
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for the determination of 23-acetate alisol B in Longdan Xiegan honey pills by HPLC. Methods:The analysis was performed on a Waters Symmetry C18 (250 mm × 4. 6 mm,5μm) column with the mobile phase of acetonitrile-0. 1% phosphoric acid (62 ∶ 38). The flow rate was 1. 0 ml·min-1, the column temperature was 35℃ and the detection wavelength was set at 208nm. Results: The linear range of 23-acetyl alisol B was 19. 999 5- 1 999. 9500 ng(r =0. 999 9), and the average recovery was 95. 56%(RSD = 0. 7%, n = 6). Conclusion: The method is simple, rapid and accurate, and can be used to control the quality of Longdan Xiegan honey pills with good repeatability and recovery.
