Effect of active ingredient from Fructus Corni on aging astrocyte induced by D-galactose
10.3969/j.issn.1001-1978.2014.12.014
- VernacularTitle:山茱萸活性成分对 D-半乳糖致衰星形胶质细胞影响的实验研究
- Author:
Yanxia MA
;
Mingyan WANG
;
Zequn JIANG
;
Fengming ZHAO
;
Aiwu HANG
- Publication Type:Journal Article
- Keywords:
Fructus Corni;
loganin;
morroniside;
D-galactose;
astrocyte;
protection
- From:
Chinese Pharmacological Bulletin
2014;(12):1688-1691,1692
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study effects of loganin and morro-niside on aging astrocytes induced by D-galactose. Methods Cortex astrocytes of newly born rats were cultured in vivo and indentified by immunofluorescence method.Firstly,appropriate D-galactose concentration was selected and effects of loganin and morroniside on proliferation activity of aging astrocytes induced by D-galactose were determined by MTT test.Then SOD, MDA were taken as indicators to study the effects of loganin and morroniside on aging astrocytes induced by D-galactose.Thirdly,growth factors like gliar cell line-derived neurotrophic factor (GDNF),basic fibro-blast growth factor (bFGF)tested by ELISA method and expression of Bax,caspase-3,phospho-extracellu-lar signal-regulated kinese (p-ERK1 /2),phospho-mi-togen-actived protein kinase /extracellular signal-regu-lated kinase kinase (p-MEK1 /2)were taken as indi-cators to discuss the potential protection mechanism. Results Loganin and morroniside exerted certain effects on proliferation of aging astrocytes induced by D-galactose,improving SOD,GDNF,bFGF release and lowering MDA release significantly (P <0.05 ). The expressions of Bax and caspase-3 proteins had no difference between model group and loganin group, morroniside group.While the expressions of p-ERK1 /2,p-MEK1 /2 of loganin group,morroniside group were improved significantly compared with model group.Conclusion Loganin and morroniside have protective effects on aging astrocytes induced by D-ga-lactose,and increase the proliferation ability.Protec-ting their antioxidant systems and improving the expres-sion of p-ERK1 /2,p-MEK1 /2 proteins are possible mechanisms.
