Antibacterial and nematicidal activity of endophyte ThF-11 of Tripterygium hypoglaucum (Levl.)Hutch
10.3969/j.issn.1001-1978.2014.12.018
- VernacularTitle:昆明山海棠内生真菌ThF11化学成分抗菌杀线虫作用的研究
- Author:
Xi ZHENG
;
Guohong LI
;
Xin WANG
;
Yan QI
;
Chunping WAN
- Publication Type:Journal Article
- Keywords:
T.hypoglaucum (Levl.)Hutch;
endo-phyte;
nematicidal;
antibacterial;
Penicillium simpli-cissimum;
chemical component;
medicinal plant
- From:
Chinese Pharmacological Bulletin
2014;(12):1709-1712,1713
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the chemical compo-nents and antibacterial and nematicidal activity of en-doparasitic fungi ThF-1 1 of Tripterygium hypoglaucum (Levl.)Hutch.Methods The endophyte ThF-1 1 was isolated,purified and identified based on fungal ITS sequence.The chemical components were isolated and identified with column chromatography and NMR spec-trometer,respectively.The nematicidal and antibacte-rial activity was investigated by biological active meth-od and inhibition zone method.Results The endo-phyte ThF-1 1 was identified as Penicillium simplicissi-mum,the compounds were identified as Penicillic acid [1 ],2,4-dimethoxy-6-methylphenol [2]and 5 (6)-dihydropenicillic acid [3 ] based on spectral data. Compound [1 ]showed significant activity against Cae-norhabditis elegans in a dose-dependent manner,and had antibacterial activity to Escherichia coli,Bacillus cereus,Bacillus subtilis, Staphylococcus aureus and Pseudomonas aeruginosa.Conclusion These com-pounds are firstly isolated from endophyte ThF-1 1 of T. hypoglaucum (Levl.)Hutch.Compound [1 ]shows strong nematicidal activity and antibacterial activity, and is worth further studying and developing.
