Protection action of resveratrol on cardiomyocyte hypertrophy mediated by endoplasmic reticulum stress

VernacularTitle:内质网应激介导白藜芦醇对心肌细胞肥大的保护作用
Author: Yan LIN ; Wei XIAO ; Li JIN ; Zhihui DENG ; Bo LI ; Guozhong WANG ; Jicheng LIU
Publication Type:Journal Article
Keywords: cardiac hypertrophy; endoplasmic reticu-lum stress; resveratrol; apoptosis; GRP78; CHOP
From: Chinese Pharmacological Bulletin 2014;(12):1721-1724,1725
CountryChina
Language:Chinese
Abstract: Aim To explore the role of resveratrol (Res)on cardiomyocyte hypertrophy induced by iso-proterenol (ISO)and the relationship with endoplas-mic reticulum stress (ERS).Methods Hypertrophic model of cardiomyocytes was induced by ISO.Hyper-trophy status of cardiomyocytes was determined by measuring the cell surface area and the gene expression of ANP.The value of apoptosis was measured by flow cytometry,the content of LDH and MDA was measured in different groups,and the gene and protein expres-sions of GRP78 and CHOP were detected by real-time PCR and Western blot.Results Res could attentuate ISO-induced cardiomyocyte hypertrophy and apoptosis by reducing the cell surface area,the gene expression of ANP and the value of apoptosis.Res could inhibit ERS by downregulating the gene and protein expression of GRP78 and CHOP.Meanwhile,the content of LDH and MDA was decreased.Conclusions The results suggest that treatment of Res may protect cardiomyocyte hypertrophy,which is partially mediated by inhibiting the expression of ERS factors GRP78 and CHOP.