A comparative study of liquid based cytology examination and DNA quantitative analysis in 879 women
10.3969/j.issn.1673-4130.2014.23.005
- VernacularTitle:879例女性宫颈液基细胞学检查与DNA定量分析法的对比研究
- Author:
Xuemei FAN
;
Wei XU
;
Jianxiang GENG
;
Xiumei ZHANG
;
Yuying ZHONG
;
Weimin CAI
;
Jing MEI
;
Haiyan XU
;
Xue ZHAO
- Publication Type:Journal Article
- Keywords:
cervical lesion;
liquid based cytology;
DNA quantitative analysis;
fully automated DNA ploidy analysis
- From:
International Journal of Laboratory Medicine
2014;(23):3165-3167,3168
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the application and clinical significance of the liquid based cytology examination and the DNA quantitative analysis in female cervical lesions.Methods The cervical cell samples were collected from 879 women participating in the comparison by the cervical brush and performed the the liquid-based thin layer section preparation for conducting Papanicolaou staining and DNA staining respectively.The liquid based cytology examination was performed on the Papanicolaou staining section and the fully automatic scanning diagnosis was performed on the DNA staining section.Results The cases of above atypical squa-mous cells of undetermined significance(ASCUS)detected by the liquid based cytology examination and the partial cases of hetero-ploid cell detected by the fully automated DNA ploidy analysis system were recommended to further perform colposcopy and cervi-cal biopsy.28 women were performed the pathological biopsy.With the cytological examination result as the standard,the detection rate of above ASCUS cervical lesions detected by the cellular DNA quantitative analysis was calculated.Conclusion The combined application of the cellular DNA quantitative analysis method and the liquid based cytology examination can obviously increase the positive detection rate of cervical cancer and precancerous lesion,which has important significance for the prevention and treatment of female cervical cancer in our country.
