Clinical application and evaluation of 4 kinds of siphilis serological detection method
10.3969/j.issn.1673-4130.2014.23.040
- VernacularTitle:4种梅毒血清学检测方法的临床应用及评价
- Author:
Jing YIN
;
Guihong XU
;
Yan LIU
- Publication Type:Journal Article
- Keywords:
Treponema Pallidum antibody;
Treponema Pallidum,i.e.,Treponema Pallidum enzyme-linked immunosorbent as-say;
Treponema Pallidum rapid plasma reagin test;
Treponema Pallidum particle agglutination test;
Treponema Pallidum anti-body detection
- From:
International Journal of Laboratory Medicine
2014;(23):3244-3245,3248
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the value of four kinds of commonly used serological detection method for detecting Trepone-ma Pallidum,i.e.,Treponema Pallidum enzyme-linked immunosorbent assay(TP-ELISA),Treponema pallidum particle agglutina-tion test(TPPA),Treponema Pallidum rapid plasma reagin test(RPR)and Treponema Pallidum antibody detection(TP-AD,emul-sion method).Methods 5 870 specimens from outpatients and inpatients were screened by TP-ELISA.121 cases of detected posi-tive specimen were simultaneously detected by TP-AD,TPPA and RPR.Then the detection results were performed the comparative analysis.Results Among 5 870 specimens,121 cases were positive by ELISA,the detection rate was 2.06%.Among 121 positive cases,119 cases were positive by TPPA,the conformity degree was 98.34%,49 cases were positive by RPR,the conformity degree was 40.41%,113 cases were positive by TP-AD,the conformity degree was 93.38%.With the TPPA results as the standard,there was no statistically significant difference between TPPA and TP-AD(P >0.05),but there was statistically significant difference be-tween TPPA and RPR(P <0.01).Conclusion The four kinds of method have their applicability.ELISA d has good specificity and high sensitivity,and is simple to operate and suitable for the physical examination of large amount of pregnant women,parturients and normal people.TPPA has good specificity and high accuracy,is suitable for definite diagnosis.RPR is suitable for the monito-ring and the curative effect observation in the patients with the active stage of siphilis.Compared with ELISA,TP-AD has slightly less sensitivity,but good specificity and can be used for screening without specific instrument.
