The effect of dienogest on apoptosis of the ectopic endometrial stroma cells and its mechanism
10.3969/j.issn.1671-8348.2014.33.006
- VernacularTitle:地诺孕素对异位子宫内膜间质细胞凋亡的影响及其机制研究
- Author:
Xiaojuan LIU
;
Yuhong ZHANG
;
Hong WANG
- Publication Type:Journal Article
- Keywords:
endometriosis;
dienogest;
apoptosis;
NF-κB
- From:
Chongqing Medicine
2014;(33):4434-4435,4439
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of dienogest on apoptosis of ectopic endometrial stroma cells and its mechanism . Methods We examine ectopic endometrial stroma cell proliferation induced by dienogest with MTT assay ,cell apoptosis by flow cytometry method and check NF‐κB ,Bcl‐2 and Bcl‐XL protein levels by Western Blot assay .Results MTT assay shows that inhibi‐tion cell proliferation induced by dienogest on ECSC is stronger than NSCS cells(P<0 .05) .Flow cytometry test shows apoptosis induced by dienogest on ECSC is stronger than NSCS cells(P<0 .05) .Western blot shows a significant reduction induced by dien‐ogest in ECSC cells of NF‐κB ,Bcl‐2 and Bcl‐XL protein levels .Conclusion Dienogest can effectively inhibit ectopic endometrial stroma cell proliferation and induce cell apoptosis by reducing expression of NF‐κB and attenuating levels of anti‐apoptotic factors such as Bcl‐2 and Bcl‐XL .
