AAV-HGFK1 inhibits proliferation of colorectal cancer cells by down-regulating the phosphorylation of EGFR
10.3969/j.issn.1671-8348.2014.33.010
- VernacularTitle:AAV-HGFK1抑制EGFR磷酸化拮抗大肠癌细胞生长
- Author:
Feihong DENG
;
Biao NIE
;
Junhua ZUO
;
Xuehua LIU
;
Jinmin CHEN
- Publication Type:Journal Article
- Keywords:
receptor,epidermal growth factor receptor;
colorectol neoplasms;
hepatocyte growth factor K1;
adeno associated virus
- From:
Chongqing Medicine
2014;(33):4447-4449,4453
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of adeno associated virus hepatocyte growth factor K1(AAV‐HGFK1)on the prolifer‐ation of 4 different colorectal cell lines with or without KRAS or BRAF mutation .Methods The levels of epidermal growth factor receptor (EGFR) mRNA were determined in SW48 without KRAS or BRAF mutation ,Lovo with KRAS mutation ,SW620 with KRAS mutation ,HT29 with BRAF mutation by quantitative real time PCR ,respectively .After the infection of AAV‐HGFK1 ,the expressions of EGFR ,p‐EGFR and β‐actin were detected by Western blot and the proliferation of the cells were assayed using MTT .Results Lovo ,SW48 and HT29 expressed EGFR protein while SW620 did not .EGF promoted the proliferation of Lovo , SW48 and HT29 cells .AAV‐HGFK1 down‐regulated the phosphorylation of EGFR and significantly inhibited their proliferation . But EGF had no effect on proliferation of SW620 stimulated by EGF .Conclusion AAV‐HGFK1 exhibited its antitumor effects through EGF/EGFR signaling irrespective of the KRAS or BRAF mutation and may also act through other signaling pathways .
