The effect of S100A4 siRNA on the inflammation and expression of TNF-α,IL-1β in adjuvant arthritis rats
10.3969/j.issn.1671-8348.2014.31.015
- VernacularTitle:S100A4 siRNA 对佐剂性关节炎大鼠炎症及细胞因子的影响
- Author:
Ganping BAI
;
Jingyi LI
;
Ronghua ZHANG
;
Yong WANG
;
Yongfei FANG
- Publication Type:Journal Article
- Keywords:
RNA,small interfering;
rats;
arthritis,experimental;
tumor necrosis factor-alpha;
interleukin-1 beta;
S100A4
- From:
Chongqing Medicine
2014;(31):4162-4164,4168
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of S100A4 siRNA on the expression of serum TNF‐α,IL‐1βand VEGF in adjuvant arthritis rats .Methods Adjuvant arthritis rat models were established and were randomly divided into model group and interfere group .On Day 11 ,rats in interfere group were injected with S100A4 siRNA fragment in articular cavity .Arthritis index (AI) chan‐ges and pathological changes of ankle joint were observed .The levels of serum TNF‐α and IL‐1β ,VEGF were detected by ELISA . Results Compared with that of model group ,the levels of serum TNF‐ α ,IL‐1β and VEGF were reduced significantly in interfere group (P< 0 .05) ;variances of AI and pathological scores in interfere group were diminished significantly (P< 0 .05) .Conclusion Inhibition of the expression of S100A4 gene can significantly reduce the expression of inflammatory factor TNF‐α ,IL‐1 β and angio‐genesis factor VEGF ,and improve the pathological injury of synovial membrane .
