microRNA-27a regulates maturation process and cytokine in LPS-stimulated dendritic cells
10.3969/j.issn.1000-484X.2015.01.006
- VernacularTitle:microRNA－27a 对树突状细胞表型及功能的影响
- Author:
Xinyou LIU
;
Yangchun ZHOU
;
Yao WANG
;
Xiaojun YANG
;
Lizong SHEN
;
Wenxi WU
- Publication Type:Journal Article
- Keywords:
microRNA-27a;
Bone marrow-derived dendritic cell;
Maturation;
Cytokine
- From:
Chinese Journal of Immunology
2015;(1):31-35,39
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of miR-27a on the phenotype and cytokine secretion in LPS-stimulated dendritic cells.Methods:Murine bone marrow-derived dendritic cells were transfected with miR-27a mimics and negative control mimics,and then stimulated by LPS for 24 hours.Dendritic cells exposed to LPS were collected for analysis of the DC immunophenotype by flow cy-tometry and supernatants were collected to determine cytokine lever.Moreover,the capability of stimulating allogeneic CD4 T+cell prolif -eration was measured by MLR ( mixed lymphocyte reaction) .Results: The levels of MHCⅡ, CD80, and CD86 were significantly increased in LPS-stimulated dendritic cells when compared with imDC ( P<0.001).Transfection with miR-27a mimics resulted in sig-nificantly lower expression levels in levels of MHCⅡ,CD80,and CD86 (P<0.001).For cytokine secretion,transfection with miR-27a mimics enhanced IL-10 production (P<0.01) and reduced the production of IL-12 (P<0.05).For MLR,transfection with miR-27a mimics suppressed allogeneic CD4+T cell proliferation.Conclusion: MiR-27a may play critical roles in regulating the maturation process and cytokine secretion in LPS-stimulated dendritic cells.
