Establishment of chip technology-based real-time PCR platform and its application in rapid detection of HCV and HIV-1
10.3969/j.issn.1673-4130.2014.22.039
- VernacularTitle:基于芯片点样技术的RT-PCR平台建立及其在HCV、HIV-1快速检测中的应用
- Author:
Bin NIE
;
Weiping LU
;
Kaijian LEI
;
Xinmin LU
- Publication Type:Journal Article
- Keywords:
HCV;
HIV;
polymerase chain reaction;
gene chip
- From:
International Journal of Laboratory Medicine
2014;(22):3092-3093,3096
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the chip technology‐based real‐time PCR (RT‐PCR) platform and to apply it in the viral loads detection of HIV‐1 and HCV .Methods Based on the primers designed to aim at the conversed regions of HIV‐1 and HCV , The gene chip tube was prepared ,and the RT‐PCR reaction system was established for the simultaneous determination of viral loads .And the melting curves were used to distinguish viral species .The sensitivity and specificity of the method were estimated , and performance of the method was verified by using clinical samples .Results The specificity of the method was good .The lowest detectable limit of the detection method of HCV and HIV‐1 was 1 × 103 copy/mL .The clinical samples with viral loads around 1 × 103 -1 × 106 copy/mL could be detected accurately .Conclusion The method provides a new idea for the detection of HCV and HIV‐1 .
