A Study on HPLC Characteristic Spectrum of Cortex Moutan in Dianjiang County
10.3969/j.issn.1005-5304.2014.12.020
- VernacularTitle:垫江牡丹皮HPLC特征图谱研究
- Author:
Caifu DENG
;
Qiusheng TAN
;
Ling LI
;
Xingyu REN
;
Min LUO
;
Wenwei ZHANG
;
Mingliang SHEN
- Publication Type:Journal Article
- Keywords:
cortex moutan;
HPLC;
characteristic spectrum;
Dianjiang County
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(12):69-71,72
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an analytical method of chromatographic fingerprint of cortex moutan in Dianjiang County through HPLC;To provide reference for the quality control and general evaluation. Methods HPLC with Shim-pack VP-ODS C18 column (4.6 mm×250 mm, 5μm) was used;paeonol was taken as standard;the mobile phase was MeOH-H2O with liner gradient eluation;the delection mavelength was at 274 nm for paeonol and 230 nm for paeoniflorin;the flow rate was 1.0 mL/min. Fourteen batches of samples were analyzed to establish the fingerprint with paeonol and paeoniflorin as reference. Results The results of the 14 batches of samples and similarity evaluation showed that the similar degrees to the 10 batches among the 14 samples were between 0.968-0.998, which illustrated the good similar degrees among samples. Conclusion The established fingerprint with characteristics of stability, good reproducibility and simplicity, can be used for the quality evaluation and control of cortex moutan.
