Content Determination of Trigonelline in Wild and Cultivated Himalaica Mirabilis of Different Districts
10.3969/j.issn.1005-5304.2014.12.022
- VernacularTitle:不同产地栽培及野生喜马拉雅紫茉莉中葫芦巴碱含量测定
- Author:
Hui LIN
;
Ting ZHAO
;
Qingjia REN
;
Huiqin ZOU
;
Zhiyu REN
;
Jiahui LI
;
Lian PENG
;
Yonghong YAN
- Publication Type:Journal Article
- Keywords:
Himalaica mirabilis;
trigonelline;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(12):76-78,79
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare differences and similarities of the content of trigonelline in Himalaica mirabilis of either wild or cultivated materials from different places. Methods An HPLC method was established to determine the content of trigonelline in Himalaica mirabilis. ZORBAX XDB-CN column (4.6 mm×250 mm, 5 μm) was used, with mobile phase of acetonitrile-0.03%acetic acid (85∶15), flow rate of 0.8 mL/min, detection wavelength of 265 nm, determination wavelength of 360 nm, and column temperature of 30 ℃. Results Regression calculation was made on peak area with the reference solution concentration, and then got the regression equation A=23.409C-26.398, r=0.999 8. Trigonelline showed good linear relation with peak area among the range of 2.004-200.400 μg/mL. The average recovery of trigonelline was 99.57%, RSD=1.11%. Conclusion There was no significant difference in the content of trigonelline of either wild or cultivated materials from different places. This study laid the foundation of application of the cultivated Himalaica mirabilis.
