Study on Adjuvant Immunoactivity of Polysaccharides from Panax Japonicus
10.3969/j.issn.1005-5304.2014.12.026
- VernacularTitle:竹节参多糖的免疫佐剂活性研究
- Author:
Qianqian CUI
;
Zhiwei SUN
;
Changcheng ZHANG
;
Ting WANG
;
Zhiyong ZHOU
;
Yumin HE
;
Chaoqi LIU
;
Ding YUAN
- Publication Type:Journal Article
- Keywords:
polysaccharides from Panax japonicus;
segmented alcohol sink;
immune adjuvant;
infrared spectroscopy
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(12):88-90,91
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the adjuvant immunoactivity of polysaccharides from Panax japonicus by alcohol of different concentrations;To discuss its part with the strongest adjuvant immunoactivity. Methods Polysaccharides from Panax japonicus was sunk with alcohol of different concentrations, and 30%alcohol compound, 60%and 90%alcohol polysaccharide were obtained. Different segments of polysaccharide and OVA protein were injected to mice once a week for three times for immunity. Five days after the last immunity, the mice were executed to collect blood, and the antibody titer was determined. The three parts of alcohol compound were scanned by infrared spectrum to determine the type of polysaccharide preliminarily. Results Compared with the control group, the antibody titer of different segments of polysaccharide obviously increased, especially the polysaccharide sunk by 60%alcohol. Infrared spectrum analysis showed that polysaccharides from Panax japonicus contained pyranose ring structure. Conclusion Polysaccharides from Panax japonicus has significant adjuvant immunoactivity, and polysaccharide sunk by 60%alcohol has the strongest adjuvant effects.
