Induced transformation of rat oval cells line WB-F344 and tumor formation in NOD/SCID mice
10.13315/j.cnki.cjcep.2014.12.007
- VernacularTitle:大鼠肝卵圆细胞系WB-F344恶性转化模型的构建及NOD/SCID小鼠成瘤实验
- Author:
Man SHU
;
Minghui ZHANG
;
Xiaoxiao HE
;
Li DING
;
Yuan LIN
;
Qinghua CAO
;
Zheng YANG
;
Ling XUE
- Publication Type:Journal Article
- Keywords:
oval cells;
malignant transformation;
tumor formation;
NOD/SCID mice
- From:
Chinese Journal of Clinical and Experimental Pathology
2014;(12):1354-1357,1364
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To study the malignant transformation after treating rat oval cell line ( WB-F344 ) with chemical carcinogen N-methyl-N′-nitro-N-nitrosoguanidine ( MNNG) . Methods WB-F344 cells were cultured with MNNG for severe times. The biological characteristics of induced cells were detected through the following methods:to check proliferation activity by flow cytometry analysis, to examine malignant transformation degree of induced cells by soft agar assay and tumor formation in NOD/SCID mice, and to investi-gate the transcriptional and protein levels of hepatocellular carcinoma marker GGT, GST-P by real time-PCR. Results Oval cells in-duced by MNNG showed changes in biological characteristics and malignant molecular markers. Conclusion Hepatic oval cells model is successfully established, which can be confirmed by tumor formation in NOD/SCID mice.
