Initial investigation on effect of breviscapine on triglyceride in HepG2 cells in different time points and its mechanism
10.3969/j.issn.1671-8348.2014.36.002
- VernacularTitle:灯盏花素在不同时间点对 HepG2肝细胞 TG含量的影响及机制探讨
- Author:
Yan YANG
;
Lin GAO
;
Huacong DENG
;
Yonghui YAN
;
Xianwen LI
;
Xiaoying WANG
;
Xin LIAO
;
Han ZHANG
;
Qirong CHEN
;
Qian WANG
- Publication Type:Journal Article
- Keywords:
breviscapine;
peroxisome proliferator-activated receptor-alpha;
apolipoprotein A5;
triglyceride
- From:
Chongqing Medicine
2014;(36):4852-4853,4856
- CountryChina
- Language:Chinese
-
Abstract:
Objective To initially investigate the time - dependent relation between breviscapine with peroxisome proliferator‐ac‐tivated receptor‐alpha(PPAR‐α) ,apolipoprotein A5 (apoA5) and triglyceride(TG) in HepG2 cells in different time points by ob‐serving the effect of breviscapine on the expression and contents of PPAR‐α ,apoA5 and TG in order to lay a certain foundation for further exploring the concrete mechanism for its regulating TG metabolism .Methods On the basis of earlier stage experiment ,100 mmol/L breviscapine was selected to treat the HepG2 cells at different time points (0 ,6 ,12 ,24 ,36 ,48 h) .The levels of PPAR‐αand apoA5 gene and protein ,and the TG content in HepG2 cells were detected .Results Breviscapine could increase the levels of PPAR‐α and apoA5 gene and protein and decrease the TG content in HepG2 cells (P< 0 .05) ,moreover which showed the time -dependence .Conclusion Breviscapine may decrease the TG level in HepG2 cells ,its mechanism may be realized by increasing the expression of PPAR‐α ,thus increacing the expression of apoA5 in HepG2 cell .
