Comparison of real time PCR and PCRˉreverse dot blot hybridization for detection of Human papillomavirus
10.3969/j.issn.1673-4130.2014.24.032
- VernacularTitle:实时PCR和PCR-RDB法检测人乳头瘤病毒的比对研究
- Author:
Kelin XIAO
;
Zehao YAN
;
Mingyue LUO
;
Guangxing MAI
;
Xi CHEN
;
Likuan XIONG
- Publication Type:Journal Article
- Keywords:
human papillomavirus;
real time polymerase chain reaction;
polymerase chain reaction-reverse dot blot hybrid-ization;
genotype
- From:
International Journal of Laboratory Medicine
2014;(24):3373-3374,3376
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare real time PCR with PCR-reverse dot blot hybridization (PCR-RDB)for detecting human pap-illomavirus (HPV)infection in women.Methods A total of 109 genital specimens from women were collected in the study.All specimens were tested HPV by using real time PCR and PCR-RDB,discrepant samples were tested again by PCR-xMAP.Results The concordant rate was 83.5%(91/109)between real time PCR and PCR-RDB (kappa=0.671),the other 18 discrepant samples were retested by PCR-xMAP,7 of those were identical with real time PCR and 11 with PCR-RDB.No differences of PCR-RDB pos-itive rates were found between the high and low viral load groups (χ2 =1.476,P =0.224).Conclusion It demonstrated moderate consistency between real time PCR and PCR-RDB.The HPV positive rates of PCR-RDB were stable when the viral loads were 103-108 .
