Expression of EBNA-1 in EB virus-induced lymphomas
10.3969/j.issn.1006-5725.2014.21.015
- VernacularTitle:EB病毒诱发淋巴瘤中EB病毒核抗原－1的表达
- Author:
Suli LU
;
Jie HE
;
Yunlian TANG
;
Runliang GAN
- Publication Type:Journal Article
- Keywords:
Herpesvirus 4,human;
Induced lymphoma;
EBNA-1
- From:
The Journal of Practical Medicine
2014;(21):3408-3410,3411
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the different expression of EBNA-1 in the normal human lymphocytes and in the EBV-induced lymphomas so as to provide the basis for studying the mechanism of the EB virus-induced lymphoma. Methods Animal model of EBV-induced lymphoma was constructed in SCID mice. EBNA-1 gene expression level was detected by real-time PCR and Western blot. Results EBV-induced lymphomas in animal model were replicated. EBNA-1 gene was expressed both in normal human lymphoma cells and 3 EBV-induced lymphoma cells. In EBV-induced lymphoma cells , the expression was up-regulated 11 683 times than in normal lymphocytes. Western blot results showed that the expression of EBNA-1 protein in induced lymphoma cells increased significantly compared with that in normal lymphocytes. Conclusions EBNA-1 expression in EBV-induced lymphoma cells is up-regulated than in normal lymphocytes. EBNA-1 may play an important role in the development of EBV-induced lymphoma.
