Expression of Tim-3 in early stages of Echinococcus granulosus infection in mice
10.3969/j.issn.1000-484X.2014.12.007
- VernacularTitle:细粒棘球蚴感染小鼠早期阶段Tim-3表达的初步分析
- Author:
Fangjie XU
;
Shuanghong YIN
;
Jun HOU
;
Hairui FANG
;
Hongqun JIANG
;
Xiangwei WU
;
Xueling CHEN
- Publication Type:Journal Article
- Keywords:
Echinococcus granulosus;
Tim-3;
TLR4;
Macrophages
- From:
Chinese Journal of Immunology
2014;(12):1616-1621,1626
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the expression levels of Tim-3,a new proinflammatory factor in the early stages of Echinococcus granulosus infection in mice.Methods: BALB/c mice were infected with E.granulosus.Peritoneal macrophages and spleen cells were collected at 1,5,9 and 13 days post-infection.At different time points ,the levels of Tim-3 in peritoneal macrophages and spleen CD3+lymphocyte subsets were detected by FCM , and the relative expression of TLR 4 mRNA was detected by qRT-PCR.Results:There was no significant difference in the expression levels of Tim-3 of CD3+spleen lymphocyte subsets between E.granulosus group and control group (P>0.05).The expression levels of Tim-3 of spleen macrophages (9,13 days) and peritoneal macrophage (5,9,13 days) were much higher in E.granulosus infected group than those in control group with statistical significance (P<0.05).The numbers of macrophages were no change.Compared with control groups,the relative expression of TLR4 mRNA at 1 day post-infection was statistically higher in E.granulosus infected group ( P<0.05 ).Conclusion:During early stage of E.granulosus infection in mice,the levels of Tim-3 expression are upregulated,while the expression of TLR4 are downregulated,which may inhibit the function of macrophages resulting in host-immunity-defensive-system inhibition and immune tolerance of E.granulosus to host.
