Influence of immune function in tumor-bearing mice by CecropinXJ
10.3969/j.issn.1000-484X.2014.12.009
- VernacularTitle:新疆家蚕抗菌肽CecropinXJ对荷瘤小鼠免疫系统的影响
- Author:
Lijie XIA
;
Yanling WU
;
Su KANG
;
Fuchun ZHANG
- Publication Type:Journal Article
- Keywords:
Antimicrobial peptides;
Cytokines;
Immune response;
Anti-tumor
- From:
Chinese Journal of Immunology
2014;(12):1627-1632,1636
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Antimicrobial peptides ( AMPs ) are peptides generated in the biological defense system against exogenous pathogens ,which is all important constituent of the non-specific immune system.The immune system can react and sometimes play an important role in tumor control both in animal models and in humans.In this study,CecropinXJ were used in the BALB/c Eca109 cells-bearing mice model.To investigate whether CecropinXJ exert anti-tumor efficiency through regulating immune response when treated with tumor-bearing mice.Methods:The expression of cytokines and DC surface molecules were detected by immunohisto-chemistry and Flow cytometry.Results:The experiment of mice showed that CecropinXJ could significantly inhibit the proliferation of transplanted tumors in dose-dependent manner.There were significant difference between control group and 10mg/kg CecropinXJ group on the volume and weight of tumor ( P<0.05 ).CecropinXJ inhibited Eca 109 cells infiltrating growth in tumor tissue without affecting viscera,and the expressions of VEGF and bFGF proteins were higher than those in the control group .The expression of cytokines and DC surface molecules,the results that,compared with the control group,CecropinXJ could not significantly promote the expression of CD4,IL-4 and IFN-γ,while not involving the expression of surface molecules on DCs in spleen (P>0.05).In CecropinXJ treatment group,TNF-αlevel in serum was significantly higher than the control group ( P<0.01 ).Conclusion: CecropinXJ could significantly inhibit tumor growth in Eca109 cells-bearing mice,and might not be affected anti-tumor efficiency through regulating immune response.
